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¹ Çukurova Üniversitesi Su Ürünleri Fakültesi, 01330 Balcalı, ADANA DOI : 10.17693/yunusae.v15i21955.235740 Viewed : 3174 - Downloaded : 2321 In this research, in aquatic environments negative effects which may occur in order to have information about, the acute toxicity insecticides Lambda-Cyhalothrin and Cypermethrin which widely used in agriculture in Cukurova Region has been researched into larvae Oreochromis niloticus (L., 1754). In this study, methods of bioassay test which called static method has been applied. The study was carried out under laboratory conditions at 25±2 ºC. The results has been estimated with the dose-response data were fitted a log-logistic model. The 24-h acute LC₅₀ values were calculated. According to the results, acute toxic effects researched Lambda-Cyhalothrin and Cypermethrin the 24-h LC₅₀ acute toxic lethal concentration values for Oreochromis niloticus (L., 1754) larvae were calculated Lambda-Cyhalothrin LC₅₀ 6.80±0.63 μgl^-1 and Cypermethrin LC₅₀ 15.88±0.65 μgl^-1. Keywords : Ýnsecticide, Lambda-Cyhalothrin, Cypermethrin, Oreochromis niloticus, Lc₅₀.