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DOI : 10.17693/yunusae.vi.235795 Viewed : 3579 - Downloaded : 1484 The karyotypic characteristics of Chalcalburnus mossulensis (Heckel, 1843) have been investigated by examining metaphase chromosomes spreads obtained from gill and kidney tissues. The fish used in the study were caught with fishing nets from two main tributaries of the Karasu River. The live fish were transported to the laboratory, and kept in a well aerated aquaria before analysis. Kidney and gill epithelia of fish were used for karyotype analysis. The best treatment parameters for preparing good metaphase chromosome spreads from the cells were optimized as hypotonic 50 minutes (0.075 M KCl) treatment, fixation with carnoy solution at 3:1 ratio (methanol: acetic acid) and a concentration of 5% Giemsa for 35 minutes. It was determined that Chalcalburnus mossulensis had 2n=50 chromosomes by investigation of the chromosome spreads. Chalcalburnus mossulensis karyotypes were determined as being composed of 8 metacentric, 10 submetacentric, 3 subtelocentric and 4 telocentric chromosome pairs (16M+20SM+6ST+8T). The fundamental arm numbers of chromosomes were determined as FN=92. Karyotype symmetry/asymmetry index was 2.12. Karyotype of gill and kidney cells was the same. No sex chromosomes were cytologically detected in the fish species. Keywords : Freshwater silver fish,chromosome,karyogram,ideogram