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¹ Cryobiology Laboratory, Aquaculture Production and Environment Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-751002, Orissa, India
² Redox Biology and Proteomics Laboratory, Department of Zoology, Center of Excellence in Environment and Public Health, Ravenshaw University, Cuttack DOI : 10.4194/AQUAST1403 Viewed : 523 - Downloaded : 364 Effect of priming on the maturation and spawning were tested in two fish species using gonadotropin-releasing hormone (GnRH), carp pituitary homogenate (CPH) and clomiphene citrate (clomid). The fish were injected with a dose of GnRH, CPH and clomid, calibrated to contain 0.3ml/kg, 7 mg/kg and 70mg/kg respectively in three split doses (two priming doses and one final dose). Two priming doses enhanced the maturation and spawning in fish as evident from hormone assay, histology and gamete analysis. Among the inducing agents, two priming doses of GnRH showed 94% maturation and higher GSI (5.31♂;18.5♀) than CPH and clomid injected rohu. Subsequently, successful spawning recorded supported by elevated levels of FSH and LH in blood. Priming with CPH and clomid showed better maturation rate than GnRH injected Nile tilapia. The sperm count of tilapia administered with CPH and clomid was 3.7±0.72×109 and 4.1±0.86×109 respectively. The efficacy of hormonal priming in synchronising the gamete development in rohu and tilapia was further evident from the increased number of synchronised oocyte development. This study may help in developing strategies for broodstock and hatchery management of carps and tilapias. Keywords : Priming Hormone Profile Maturation L. rohita O. niloticus