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¹ İnönü University, Faculty of Fishery, Department of Aquaculture, 44280, Malatya, Turkey DOI : 10.4194/2618-6381-v18_2_01 Viewed : 9647 - Downloaded : 3362 This study was aimed to determine the effects of Na+, K+ and Ca2+ ion-based different sperm activator medias (SAM) on the velocity and movement style of Brown trout (Salmo trutta) sperm cells. SAMs were prepared such as SAM1 (9 mM NaCl, 50 mM Glycine and 20 mM Tris), SAM2 (103 mM NaCl, 40 mM KCl, 20 mM HEPES), SAM3 (125 mM NaCl, 30 mM Tris), SAM4 (60 mM NaHCO3, 50 mM Tris) and SAM5 (125 mM NaCl, 1 mM CaCl2, 30 mM Glycine, 20 mM Tris), respectively. It was kept at the same pH: 9.0 for all activators. According to the study data, the statistically significant increases (P<0.05) were found in the velocities of sperm cells in SAM5 activator compared to other activators. All activators showed an effect of increasing and decreasing on the values of linearity and amplitude of lateral displacement of the spermatozoa head in movement style of sperm cells but these were found to be statistically insignificant (P>0.05). As a result, unlike other activators, the presence of the Ca2+ (1 mM) ion in the SAM5 activator has resulted in an increase in the velocities of sperm cells in brown trout sperm cells. Keywords : Salmo trutta, Velocity and movement style, Sperm activator media