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¹ Central Fisheries Research Institute 61250 Yomra, Trabzon, Turkey
² İstanbul Üniversitesi Su Ürünleri Fakültesi Laleli, İstanbul, Turkey DOI : 10.17693/yunusae.v2012i21908.235432 Viewed : 3645 - Downloaded : 1966 In this research, milt collection and cryopreservation with different extenders in endangered Russian sturgeon (Acipencer gueldenstaedtii) which lives Black Sea and its tributary rivers were studied. The semen was collected by abdominal massage. Sperm motility classified to five categories. Cooling were carried out as follow; 3°C/min between 0 and -4 and 5°C/min between - 4 and -10. Sperm were frozen using 5 different extenders and with 8% DMSO as cyroprotectant at -196 °C in liquid nitrogen. Spermatocrite ratios range from 65% to 91%. Sperm pH was determined 8.0. After 24 hours the deep frozen sperm samples were thawed by quickly immersing them in a water bath at 32 ºC under constant shaking for about 2 min, and freshwater was added to stimulate the sperm motility. In ten minutes after activation, the velocity and motility of sperm were ++ and 20%. Our results indicate that E2 extender was better than the others and may useful extender for cryopreservation of sturgeon semen. Keywords : Russian sturgeon, semen collection, semen evaluation, Cryopreservation