Aquaculture Studies 2017, Vol 17, Num, 3     (Pages: 283-294)

Automatic Cells Counting in Natt-Herrick Stained Fish Blood

M. Ozan İncetaş 1 , Erdinç VESKE 2 ,Nesrin EMRE 3 ,Recep DEMİRCİ 4

1 Bülent Ecevit University, Engineering Faculty, Computer Eng. Dep., Zonguldak-Turkey
2 Republic of Turkey Ministry of Food, Agriculture and Livestock Ankara-Turkey
3 Mediterranean Fisheries Research, Production and Training Institute, Antalya-Turkey
4 Gazi University, Technology Faculty, Computer Eng. Dep., Ankara-Turkey
DOI : 10.17693/yunusae.v17i30729.306490 Viewed : 494 - Downloaded : 205 Monitoring of hematological values which provide important information about the health status of fish is considerably important in aquaculture. One of the most commonly used methods for detecting the hematological values in fish blood is the usage of Natt-Herrick solution. Basically, in this approach, Natt-Herrick stained blood samples are examined with a microscope and the cells are counted. Nevertheless, the counting process is both tough and time-consuming. In this study, a technique in which cell counting in blood samples images is automatically performed has been presented. Natt-Herrick stained blood samples of Oncorhynchus mykiss and Sparus aurata were used for evaluation of the developed scheme. The outputs generated by automatic blood cells detection algorithm in 90 images were compared with results which were obtained by means of user`s intervention. Consequently, an average f-score over 0.96 was achieved. Keywords : Fish blood, Natt-Herrick solution, Automatic cell counting